Intracellular neutral carrier-based Ca2+ microelectrode with subnanomolar detection limit

Intracellular K(+) Determination With a Potentiometric Microelectrode Based on ZnO Nanowires

The fabrication and application of an intracellular K + -selective microelectrode is demonstrated. ZnO nanowires with a diameter of 100–180 nm and a length of approximately 1.5 μm are grown on a borosilicate glass microcapillary. The ZnO nanowires were coated by a K + -ionophore-containing membrane. The K + -selective microelectrode exhibited a K + -dependent potentiometric response versus an A...

Neutral carrier electrode for continuous measurement of blood Ca2+ in the extracorporeal circulation.

A neutral-carrier-based Ca2+-selective electrode exposed to whole blood exhibited an EMF-stability better than 0.13 mV (approximately 1% change of the initial activity) during 3.7 to 6.6 h. It has been used successfully to monitor ionic Ca2+ in the extracorporeal circulation during administration of calcitonin to dogs. Data simulation shows that the apparent concentration of ionic Ca2+ is affec...

Intracellular Ca2+ signaling

Functional intracellular Ca(2+) signaling is essential for the upregulation of the canonical mTOR-controlled autophagy pathway triggered by rapamycin or by nutrient deprivation. Moreover, modifications in the Ca(2+)-signaling machinery coincide with autophagy stimulation. This results in enhanced intracellular Ca(2+) signaling essential for driving the autophagy process. Yet, the mechanisms ups...

Beta-Thalassemia Carrier Detection by NESTROFT

FRET-Based Nanobiosensors for Imaging Intracellular Ca2+ and H+ Microdomains

Semiconductor nanocrystals (NCs) or quantum dots (QDs) are luminous point emitters increasingly being used to tag and track biomolecules in biological/biomedical imaging. However, their intracellular use as highlighters of single-molecule localization and nanobiosensors reporting ion microdomains changes has remained a major challenge. Here, we report the design, generation and validation of FR...